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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been observed in several tumors, which results in chromosome instability, and so may add to tumorigenesis. This protein was found to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a important role in marketing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are explained. Two added splice variants have been claimed but comprehensive nucleotide sequence info just isn't nevertheless available.
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Blocking peptides are peptides that bind precisely into the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified via the antibody.
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This guideline outlines even further attainable check here optimization of mobile and immunoassay parameters to make sure the absolute best results are...
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This manual outlines more attainable optimization of cellular and immunoassay parameters to be sure the best possible effects are...
Antibodies bound to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.
Focus on Facts Cyclin E belongs on the very conserved cyclin loved ones, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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